Beta-galactosidase from Lactobacillus pentosus: purification, characterization and formation of galacto-oligosaccharides.

@article{Maischberger2010BetagalactosidaseFL,
  title={Beta-galactosidase from Lactobacillus pentosus: purification, characterization and formation of galacto-oligosaccharides.},
  author={Thomas Maischberger and Elisabeth Leitner and Sunee Nitisinprasert and Onladda Juajun and Montarop Yamabhai and T U Nguyen and Dietmar Haltrich},
  journal={Biotechnology journal},
  year={2010},
  volume={5 8},
  pages={838-47}
}
A novel heterodimeric beta-galactosidase with a molecular mass of 105 kDa was purified from crude cell extracts of the soil isolate Lactobacillus pentosus KUB-ST10-1 using ammonium sulphate fractionation followed by hydrophobic interaction and affinity chromatography. The electrophoretically homogenous enzyme has a specific activity of 97 U(oNPG)/mg protein. The K(m), k(cat) and k(cat)/K(m) values for lactose and o-nitrophenyl-beta-D-galactopyranoside (oNPG) were 38 mM, 20 s(-1), 530 M(-1).s(-1… CONTINUE READING

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