Beta and gamma subunits of a yeast guanine nucleotide-binding protein are not essential for membrane association of the alpha subunit but are required for receptor coupling.

@article{Blumer1990BetaAG,
  title={Beta and gamma subunits of a yeast guanine nucleotide-binding protein are not essential for membrane association of the alpha subunit but are required for receptor coupling.},
  author={Kendall J Blumer and J. Thorner},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1990},
  volume={87 11},
  pages={4363-7}
}
Conditions were devised to demonstrate GTP-regulated coupling between the yeast STE2-encoded receptor and its cognate guanine nucleotide-binding protein (G protein). Treatment of partially purified membranes with guanosine 5'-[gamma-thio]triphosphate (GTP[gamma-S]) converted the receptor from a high-affinity state (Kd = 17 nM) to a much lower affinity state (Kd approximately 150 nM), as judged by three independent criteria: rate of ligand (alpha-factor) dissociation, equilibrium binding, and… CONTINUE READING