Beta-N-acetylhexosaminidases A and S have similar sub-cellular distributions in HL-60 cells.

@article{Martino1995BetaNacetylhexosaminidasesAA,
  title={Beta-N-acetylhexosaminidases A and S have similar sub-cellular distributions in HL-60 cells.},
  author={Simone De Martino and Carla Emiliani and Antonio Orlacchio and Ramin Hosseini and John Laing Stirling},
  journal={Biochimica et biophysica acta},
  year={1995},
  volume={1243 3},
  pages={
          489-95
        }
}
In HL-60 cells the most abundant isoenzymes of beta-N-acetyl-hexosaminidase are A (alpha beta) and S (alpha alpha). Sub-cellular fractionation of HL-60 cells by differential centrifugation showed that both A and S forms were present in the lysosomal and post-lysosomal (soluble) fractions in approximately equal abundance. Ion-exchange chromatography showed that a fraction enriched with plasma membranes had the A form, and a form of beta-N-acetylhexosaminidase less acidic than A, but there was no… CONTINUE READING