Beta Hydroxylation of Glycolipids from Ustilago maydis and Pseudozyma flocculosa by an NADPH-Dependent β-Hydroxylase

@article{Teichmann2011BetaHO,
  title={Beta Hydroxylation of Glycolipids from Ustilago maydis and Pseudozyma flocculosa by an NADPH-Dependent $\beta$-Hydroxylase},
  author={Beate Teichmann and François Lefebvre and Caroline Labbé and Michael B{\"o}lker and Uwe Linne and Richard R B{\'e}langer},
  journal={Applied and Environmental Microbiology},
  year={2011},
  volume={77},
  pages={7823 - 7829}
}
ABSTRACT Flocculosin and ustilagic acid (UA), two highly similar antifungal cellobiose lipids, are respectively produced by Pseudozyma flocculosa, a biocontrol agent, and Ustilago maydis, a plant pathogen. Both glycolipids contain a short-chain fatty acid hydroxylated at the β position but differ in the long fatty acid, which is hydroxylated at the α position in UA and at the β position in flocculosin. In both organisms, the biosynthesis genes are arranged in large clusters. The functions of… 
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References

SHOWING 1-10 OF 29 REFERENCES
Identification of a biosynthesis gene cluster for flocculosin a cellobiose lipid produced by the biocontrol agent Pseudozyma flocculosa
TLDR
By genetic and mass spectrometric analysis, it is shown that Uat2 and Fat2 are necessary for acetylation of the corresponding glycolipid.
A biosynthetic gene cluster for a secreted cellobiose lipid with antifungal activity from Ustilago maydis
TLDR
It is demonstrated that infection of tomato leaves by the plant pathogenic fungus Botrytis cinerea is prevented by co‐inoculation with wild‐type U. maydis sporidia, indicating that UA secretion is critical for antagonistic activity.
Genetic Analysis of Biosurfactant Production in Ustilago maydis
TLDR
The mutants described here allow for the first time a genetic analysis of glycolipid production in fungi and it is shown that mannosylerythritol lipid production is responsible for hemolytic activity on blood agar, whereas ustilagic acid secretion is required for long-range pheromone recognition.
Nutritional regulation and kinetics of flocculosin synthesis by Pseudozyma flocculosa
TLDR
Concentration of the start-up inoculum was found to play an important role in flocculosin production, as the optimal level increased productivity by as much as tenfold.
Cinnamoyl CoA reductase, the first committed enzyme of the lignin branch biosynthetic pathway: cloning, expression and phylogenetic relationships.
TLDR
Sequence analysis revealed remarkable homologies with dihydroflavonol-4-reductase (DFR), the first enzyme of the anthocyanin biosynthetic pathway, suggesting that CCR shared a common ancestor with these enzymes and can therefore be considered as a new member of the mammalian 3 beta-hydroxysteroid dehydrogenase/ plant diHydroflvonol reductase superfamily.
BIOCHEMISTRY OF THE USTILAGINALES: VII. ANTIBIOTIC ACTIVITY OF USTILAGIC ACID
TLDR
The antibiotic activity of Ustilago zeae cultures is associated mainly with the culture solids and it has been shown that a mixture of glucolipids, called ustilagic acid, is responsible for part, if not all, of the observed activity.
Heterologous expression of dihydroflavonol 4‐reductases from various plants
Cloning and molecular analysis of structural genes involved in flavonoid and stilbene biosynthesis in grape (Vitis vinifera L.)
TLDR
The analysis of the genomic organization and expression of these genes in response to light shows that PAL and StSy genes belong to large multigene families, while the others are present in one to four copies per haploid genome.
Mechanisms and means of detection of biocontrol activity of Pseudozyma yeasts against plant-pathogenic fungi.
Short‐chain dehydrogenase/reductase (SDR) relationships: A large family with eight clusters common to human, animal, and plant genomes
TLDR
The availability of the human genome enabled the large family of short‐chain dehydrogenase/reductase (SDR) members to be delineated, and 63 members are found, reflecting enzyme forms specific to mammals.
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