Beta A3/A1 crystallin from human cataractous lens contains an intramolecular disulfide bond.


PURPOSE To develop an experimental approach that can identify amino acid sequences containing cysteine residues involved in disulfide bonding during cataractogenesis of the human lens. METHODS Total proteins from cataractous and normal human lenses were solubilized anaerobically, followed by carboxy-methylation of free sulfhydryl groups with iodoacetate… (More)