Beta 1-6 branching of N-linked carbohydrate is associated with K-ras mutation in human colon carcinoma cell lines.

@article{Wojciechowicz1995Beta1B,
  title={Beta 1-6 branching of N-linked carbohydrate is associated with K-ras mutation in human colon carcinoma cell lines.},
  author={Donald Wojciechowicz and P Y Park and Philip B. Paty},
  journal={Biochemical and biophysical research communications},
  year={1995},
  volume={212 3},
  pages={758-66}
}
Here the K-ras genotypes of nine colon carcinoma cell lines are compared to the protein glycosylation patterns found in these cells. By a variety of methodologies utilizing lectins to probe carbohydrate structure, we find evidence that five out of six cell lines having K-ras mutations have elevated amounts of beta 1-6 branching at the trimannosyl core of N-linked carbohydrate. None of the three K-ras wild type cell lines assayed have evidence of elevated beta 1-6 branching. In five out of five… CONTINUE READING