Beta‐chemokine receptors 5 and 3 are expressed on the head region of human spermatozoon

  title={Beta‐chemokine receptors 5 and 3 are expressed on the head region of human spermatozoon},
  author={Barbara Muciaccia and Fabrizio Padula and Elena Vicini and Loredana Gandini and Andrea Lenzi and Mario Stefanini},
  journal={The FASEB Journal},
Induction of human sperm chemotaxis is an established phenomenon, though signaling systems physiologically involved have not been identified. Recently, it has been demonstrated that RANTES is present in the follicular fluid and that this molecule is a chemoactractant for human spermatozoa. However, the presence of ß‐chemokine receptors on human spermatozoa has never been reported. By cytometric, Western blotting and immunofluorescence analysis, we demonstrate the presence of CCR5 and CCR3 on… 

Beta-chemokine receptor CCR5 in human spermatozoa and its relationship with seminal parameters.

The percentage of CCR5-positive spermatozoa may vary under conditions associated with changes in membrane architecture and spermatozosa showed large intracellular pools of the receptor.

A Role for the Chemokine Receptor CCR6 in Mammalian Sperm Motility and Chemotaxis

The data indicate that chemokine ligand/receptor interactions in the male and female genital tracts promote sperm motility and chemotaxis under non‐inflammatory conditions and might find application in clinical reproduction and/or contraception.

Different locations of RANTES and its receptors on mouse epididymal spermatozoa.

RANTES and its receptors were not in the same sperm location, suggesting that RANTES binding to mouse epididymal spermatozoa is independent of CCR1, CCR3 and CCR5.

RANTES and human sperm fertilizing ability: effect on acrosome reaction and sperm/oocyte fusion.

Results indicate a negative interference of high levels of RANTES on the sperm fertilizing ability, thereby suggesting a potential contribution of this chemokine to subfertility associated with endometriosis and genital tract inflammatory diseases.

HIV-1 viral DNA is present in ejaculated abnormal spermatozoa of seropositive subjects.

Molecular evidence is reported demonstrating that HIV-1 infected subjects can ejaculate small amounts ofAIDS-1 DNA-positive abnormal spermatozoa, and their possible role in HIV- 1 sexual transmission remains to be clarified.

Receptors in spermatozoa: are they real?

The receptors for well-known hormones, growth factors, and neurotransmitters that have been reported in the literature are reviewed and their authenticity and physiologic relevance will be discussed.

Immunomodulatory effects of chemokines during the early implantation window.

This work analyzes data from both fertile women and patients having experienced recurrent spontaneous abortions as representative of physiological and pathological situations respectively for chemokines expression levels and regulation on three different levels.

Prolactin selectively inhibits the LPS-induced chemokine secretion of human foetal membranes

  • P. Flores-EspinosaR. Vega-Sánchez V. Zaga-Clavellina
  • Medicine, Biology
    The journal of maternal-fetal & neonatal medicine : the official journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians
  • 2019
PRL inhibits the differential secretion of proinflammatory chemokines by human fetal membranes in both amnion and choriodecidua regions.

Decreased Toll-like Receptor (TLR) 2 and 4 Expression in Spermatozoa in Couples with Unexplained Recurrent Spontaneous Abortion (URSA).

Studies have shown that toll-like receptors (TLRs) play some important roles in reproductive processes such as ovulation, spermatogenesis, sperm capacitation, fertilization, and pregnancy to the best

Quantification of CatSper1 expression in human spermatozoa and relation to functional parameters.

The results demonstrate an association of CatSper1 expression with human sperm progressive and HA motility and provide preliminary evidence that lack of expression or mislocalization of Catsper1 in spermatozoa may be involved in the pathogenesis of asthenozoospermia.



Detection of a short CCR5 messenger RNA isoform in human spermatozoa.

Using reverse transcription and real-time quantitative polymerase chain reaction (RQ-PCR) analysis, it is found that the CCR5 mRNA isoform in human spermatozoa consists of exons 3 and 4, and is shorter than the transcript in leukocytes.

The effect of RANTES on human sperm chemotaxis.

It was demonstrated that mRNA for the RANTES receptors CCR-1 and CCR -5 are present in human sperm and RantES is involved in the chemotactic effect of FF in vitro.

HIV-1 chemokine co-receptor CCR5 is expressed on the surface of human spermatozoa

It is demonstrated, by cytometric analysis and immunofluorescence microscopy, the presence of β-chemokine receptor 5 (CCR5) on the periacrosomal region of ejaculated spermatozoa may allow sperm to act as virion cellular carriers during the sexual transmission of HIV-1 infection.

Cellular localization of the chemokine receptor CCR5. Correlation to cellular targets of HIV-1 infection.

It is suggested that CCR5+ cells are recruited to inflammatory sites and, as such, may facilitate transmission of macrophage-tropic strains of HIV-1.

Mammalian sperm chemotaxis and its association with capacitation.

It was found that only a small fraction of a given sperm population is chemotactically responsive and that this fraction constitutes capacitated (ripe) spermatozoa, and the role of the continuous replacement of chemotactic/capacitated spermatozosa is to prolong the time during which capacitated spermutozoa are available in the female reproductive tract.

Expression of a Functional Eotaxin (CC Chemokine Ligand 11) Receptor CCR3 by Human Dendritic Cells1 2 3

It is postulated that expression of CCR3 may underlie situations where both DCs and eosinophils accumulate in vivo, such as the lesions of patients with Langerhans cell granulomatosis.

Sperm from a variety of mammalian species express beta1,4-galactosyltransferase on their surface.

Results demonstrate that various mammalian species express GalTase on their surface and that it is found in the proper location to bind to the zona pellucida.

Sperm chemotaxis.

The physiological role of sperm chemotaxis in non-mammalian species appears to differ from that in mammals, and the claim that progesterone is the attractant for human spermatozoa has failed to be substantiated, neither have claims for other mammalian sperm attractants been verified.

Chemokines and human reproduction.

Chemotaxis of Capacitated Rabbit Spermatozoa to Follicular Fluid Revealed by a Novel Directionality-Based Assay1

It is concluded that rabbit spermatozoa, like human spermatoza, are chemotactically responsive to FF factor(s) and acquire this responsiveness as part of the capacitation process.