Berberine Reduces cAMP-Induced Chloride Secretion in T84 Human Colonic Carcinoma Cells through Inhibition of Basolateral KCNQ1 Channels

  title={Berberine Reduces cAMP-Induced Chloride Secretion in T84 Human Colonic Carcinoma Cells through Inhibition of Basolateral KCNQ1 Channels},
  author={Rodrigo Alzamora and Fiona O’Mahony and Wing-Hung Ko and Tiffany Wai-Nga Yip and Derek Carter and Mustapha Irnaten and Brian J Harvey},
  journal={Frontiers in Physiology},
Berberine is a plant alkaloid with multiple pharmacological actions, including antidiarrhoeal activity and has been shown to inhibit Cl− secretion in distal colon. The aims of this study were to determine the molecular signaling mechanisms of action of berberine on Cl− secretion and the ion transporter targets. Monolayers of T84 human colonic carcinoma cells grown in permeable supports were placed in Ussing chambers and short-circuit current measured in response to secretagogues and berberine… Expand
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K2P TASK‐2 and KCNQ1–KCNE3 K+ channels are major players contributing to intestinal anion and fluid secretion
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  • Medicine
  • Frontiers in Physiology
  • 2021
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Berberine inhibits Wilms' tumor cell progression through upregulation of Wilms' tumor gene on the X chromosome.
A novel mechanism that may contribute to the antineoplastic effects of berberine which was also demonstrated by recent population studies is suggested; however, further studies are required to investigate the potential therapeutic use of Berberine in patients with Wilms' tumor. Expand
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The Actions of Ursodeoxycholic Acid and its derivatives on Colonic Epithelial Transport and Barrier Function
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Hydrogen peroxide inhibits cAMP-induced Cl- secretion across colonic epithelial cells.
It is concluded that the principal effect of H2O2on colonic Cl- secretion is inhibitory, due to a decrease in ATP levels following H1N1 treatment, which subsequently results in an inhibition of the apical membrane Cl- conductance and basolateral membrane Na+-K+-ATPase activity. Expand
Berberine inhibits ion transport in human colonic epithelia.
Berberine exerts an anti-secretory action directly upon epithelial cells and the mechanism of action may be at the level of blockade of K+ channels, indicating an inhibitory activity directly at thelevel of the epithelium rather than an indirect effect through other mucosal element(s). Expand
Berberine inhibition of electrogenic ion transport in rat colon
The precise mechanism of action of berberine remains to be elucidated, however, its site of action appears to be distal to second messenger production and may be at a level common to all stimuli of colonic chloride secretion. Expand
Levamisole inhibits intestinal Cl- secretion via basolateral K+ channel blockade.
Phenylimidazothiazoles block K+ channels required for Cl(-)-secretory responses elicited by diverse pathways in model epithelia and native colon, an effect that outweighs their ability to activate apical Cl- channels. Expand
Molecular and functional characterization of the small Ca2+-regulated K+ channel (rSK4) of colonic crypts
Data indicate that the Ca2+-activated and imidazole-inhibited basolateral K+ current in the colon is caused by SK4 channels, similar to those of the known human SK channel. Expand
The antifungal antibiotic, clotrimazole, inhibits chloride secretion by human intestinal T84 cells via blockade of distinct basolateral K+ conductances. Demonstration of efficacy in intact rabbit colon and in an in vivo mouse model of cholera.
Direct evidence is provided that CLT blocks Cl- secretion in intestinal T84 cells by inhibition of basolateral K+ conductances, and the results further support the suggestion thatCLT and its metabolites may show clinical efficacy in the treatment of secretory diarrheas of diverse etiologies. Expand
Inhibition of cAMP- and Ca-dependent Cl- secretion by phorbol esters: inhibition of basolateral K+ channels.
  • W. Reenstra
  • Chemistry, Medicine
  • The American journal of physiology
  • 1993
The results demonstrate that the primary effect of PMA on Cl secretion is not inhibition of apical Cl channels but inhibition of basolateral K channels, which is 10-fold greater in Cl media than in gluconate media. Expand
Effects of berberine on basal and secretagogue-modified ion transport in the rabbit ileum in vitro.
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Phorbol ester sequentially downregulates cAMP-regulated basolateral and apical Cl- transport pathways in T84 cells.
The effect of phorbol esters on adenosine 3',5'-cyclic monophosphate (cAMP)-regulated epithelial Cl- transport was studied in T84 cells, a human colonic cell line that serves as a model forExpand
Antisecretory effects of berberine in rat ileum.
It is clear that mucosal berberine affects intestinal ion transport by mechanisms different from stimulation of the Na pump and probably at a step distal to the production or degradation of cyclic AMP or cyclic GMP. Expand