Corpus ID: 4546453

Benzodiazepine modulation of recombinant alpha1beta3gamma2 GABA(A) receptor function efficacy determination using the Cytosensor microphysiometer.

@article{Smith1998BenzodiazepineMO,
  title={Benzodiazepine modulation of recombinant alpha1beta3gamma2 GABA(A) receptor function efficacy determination using the Cytosensor microphysiometer.},
  author={A. Smith and R. M. Mckernan and J. Atack},
  journal={European journal of pharmacology},
  year={1998},
  volume={359 2-3},
  pages={
          261-9
        }
}
  • A. Smith, R. M. Mckernan, J. Atack
  • Published 1998
  • Medicine
  • European journal of pharmacology
  • Gamma-aminobutyric acid (GABA) dose dependently increased extracellular acidification rate in Ltk cells stably expressing human recombinant alpha1beta3gamma2 GABA(A) receptors but had no effect in non-transfected controls. Cells seeded at 1 x 10(5) cells/cup, with 4-5 days induction, had basal acidification rates of 105+/-2 microVs(-1) at 37 degrees C (mean+/-standard error of mean, n=37). GABA responses had a characteristic time-course with an initial alkalinisation followed by a peak of… CONTINUE READING