Behaviour of microtubules and actin filaments in living Drosophila embryos.

@article{Kellogg1988BehaviourOM,
  title={Behaviour of microtubules and actin filaments in living Drosophila embryos.},
  author={Douglas R. Kellogg and Timothy J Mitchison and Bruce M. Alberts},
  journal={Development},
  year={1988},
  volume={103 4},
  pages={675-86}
}
We describe the preparation of novel fluorescent derivatives of rabbit muscle actin and bovine tubulin, and the use of these derivatives to study the behaviour of actin filaments and microtubules in living Drosophila embryos, in which the nuclei divide at intervals of 8 to 21 min. The fluorescently labelled proteins appear to function normally in vitro and in vivo, and they allow continuous observation of the cytoskeleton in living embryos without perturbing development. By coinjecting labelled… CONTINUE READING
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