Behavioral decoding of Drosophila locomotion in a circular arena

  title={Behavioral decoding of Drosophila locomotion in a circular arena},
  author={Shuang Qiu and R. Meldrum Robertson and Chengfeng Xiao},
Motion detection and position tracking of animal behavior over a period of time produce massive amount of information, but analysis and interpretation of such huge datasets are challenging. Here we describe statistical methods to extract major movement structures of Drosophila locomotion in a circular arena, and examine the effects of pulsed light stimulation on these identified locomotor structures. Drosophila adults performed exploratory behavior when restrained individually in the circular… 
Determination of LD50, Fecundity and Locomotor Effects of Methanol Root Extract of Ximenia americana Linn, in Drosophila melanogaster
All the tested concentrations of the methanol extract of Ximenia americana in D. melanogaster showed that the plant extract is relatively safe and slightly increase the emergence of new fly with no noticeable negative effect in locomotor activity.


Locomotion Induced by Spatial Restriction in Adult Drosophila
A protocol for high-throughput analysis of locomotion was developed and characterized features of locomotions in a restricted space and revealed that the white gene was not associated with the phenotype of boundary preference in wildtype flies.
Open Source Tracking and Analysis of Adult Drosophila Locomotion in Buridan's Paradigm with and without Visual Targets
A package of open source software designed to track a single animal walking in a homogenous environment (Buritrack) and to analyze its trajectory confirms that flies are centrophobic and shows that inaccessible visual targets can alter the orientation of the flies without changing their overall patterns of activity.
Automated Tracking of Animal Posture and Movement during Exploration and Sensory Orientation Behaviors
SOS is a simple and versatile system to track body posture and motion of single animals in two-dimensional environments and is suitable to carry out high-resolution behavioral tracking for a wide range of organisms including flatworms, fishes and mice.
Saccadic body turns in walking Drosophila
The analysis shows that Drosophila does not turn its head relative to its body when turning during walking, which contrasts with the head saccades reported for e.g., blowflies and honeybees.
Targeted expression of tetanus toxin reveals sets of neurons involved in larval locomotion in Drosophila.
The results presented here provide a basis for future genetic studies of motor control in the Drosophila larva and blocked neurotransmitter release from defined populations of neurons by targeted expression of tetanus toxin light chain (TeTxLC) with the GAL4/UAS system.
FlyLimbTracker: An active contour based approach for leg segment tracking in unmarked, freely behaving Drosophila
A method that uses active contours to semi-automatically track body and leg segments from video image sequences of unmarked, freely behaving Drosophila is described and its open-source software implementation, FlyLimbTracker, is provided, opening up the possibility of tracking leg movements in other species by modifications of underlying active contour models.
High-throughput Ethomics in Large Groups of Drosophila
A camera-based method for automatically quantifying the individual and social behaviors of fruit flies, Drosophila melanogaster, interacting in a planar arena finds that behavioral differences between individuals were consistent over time and were sufficient to accurately predict gender and genotype.
Modeling Drosophila Positional Preferences in Open Field Arenas with Directional Persistence and Wall Attraction
Differences in the two-component model for fly movement suggest that there are emergent features within these complex arenas that have significance for the fly, such as potential shelter.
Pulsed Light Stimulation Increases Boundary Preference and Periodicity of Episodic Motor Activity in Drosophila melanogaster
It is concluded that pulsed light stimulation modifies behavioral and electrophysiological activities in w1118 flies and provides a foundation for future research on the genetic mechanisms of neural plasticity underlying such behavioral modification.
Exploratory Activity in Drosophila Requires the kurtz Nonvisual Arrestin
The exploratory phase of open field activity requires kurtz in the nervous system, but is independent of dopamine's stimulation of activity.