Beam scanning for rapid coherent Raman hyperspectral imaging.

  title={Beam scanning for rapid coherent Raman hyperspectral imaging.},
  author={Ian Seungwan Ryu and Charles H Camp and Ying Jin and Marcus T Cicerone and Young Jong Lee},
  journal={Optics letters},
  volume={40 24},
Coherent Raman imaging requires high-peak power laser pulses to maximize the nonlinear multiphoton signal generation, but accompanying photo-induced sample damage often poses a challenge to microscopic imaging studies. We demonstrate that beam scanning by a 3.5-kHz resonant mirror in a broadband coherent anti-Stokes Raman scattering (BCARS) imaging system can reduce photo-induced damage without compromising signal intensity. Additionally, beam scanning enables slit acquisition, in which spectra… CONTINUE READING