Bcl-2 mRNA-targeted ribozymes: effects on programmed cell death in chronic myelogenous leukemia cell lines

@article{Scheid1998Bcl2MR,
  title={Bcl-2 mRNA-targeted ribozymes: effects on programmed cell death in chronic myelogenous leukemia cell lines},
  author={S{\'a}ndor Scheid and M. Heinzinger and Cornelius F Waller and Winand Lange},
  journal={Annals of Hematology},
  year={1998},
  volume={76},
  pages={117-125}
}
 We used synthetic RNA transcripts to prove the cleavage capability of ribozymes targeted against bcl-2-related RNAs. No cleavage occurred when control oligonucliotides were used. To assess the functional role of the specific ribozymes in chronic myelogenous leukemia (CML) cell lines we cultured K562, BV173, and Daudi cells for 48 h after lipofection with 10 μM oligonucleotide. An increase in apoptotic cells, dependent on ribozyme specificity, was shown in BV173 cells.This finding was… CONTINUE READING

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