Bayesian localisation microscopy reveals nanoscale podosome dynamics

@inproceedings{Cox2011BayesianLM,
  title={Bayesian localisation microscopy reveals nanoscale podosome dynamics},
  author={Susan Cox and Edward Rosten and James Monypenny and Tijana Jovanovic-Talisman and Dylan T. Burnette and Jennifer Lippincott-Schwartz and Gareth E Jones and Rainer Heintzmann},
  booktitle={Nature Methods},
  year={2011}
}
We describe a localization microscopy analysis method that is able to extract results in live cells using standard fluorescent proteins and xenon arc lamp illumination. Our Bayesian analysis of the blinking and bleaching (3B analysis) method models the entire dataset simultaneously as being generated by a number of fluorophores that may or may not be emitting light at any given time. The resulting technique allows many overlapping fluorophores in each frame and unifies the analysis of the… CONTINUE READING
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