Batch production of a silk-elastin-like protein in E. coli BL21(DE3): key parameters for optimisation

@inproceedings{Collins2013BatchPO,
  title={Batch production of a silk-elastin-like protein in E. coli BL21(DE3): key parameters for optimisation},
  author={Tony Collins and Jo{\~a}o Azevedo-Silva and Andr{\'e} Luis Sales da Costa and Fernando Branca and Ra{\'u}l Machado and Margarida Casal},
  booktitle={Microbial cell factories},
  year={2013}
}
BackgroundSilk-elastin-like proteins (SELPs) combining the physicochemical and biological properties of silk and elastin have a high potential for use in the pharmaceutical, regenerative medicine and materials fields. Their development for use is however restrained by their production levels. Here we describe the batch production optimisation for a novel recently described SELP in the pET-E. coli BL21(DE3) expression system. Both a comprehensive empirical approach examining all process… CONTINUE READING
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