Basta tolerance as a selectable and screening marker for transgenic plants of Norway spruce

@article{Brukhin2000BastaTA,
  title={Basta tolerance as a selectable and screening marker for transgenic plants of Norway spruce},
  author={Vladimir Brukhin and David E. Clapham and Malin Elfstrand and Sara von Arnold},
  journal={Plant Cell Reports},
  year={2000},
  volume={19},
  pages={899-903}
}
The bar gene conferring resistance to the herbicide Basta (containing phosphinothricin) was transferred to embryogenic cultures of Picea abies by particle bombardment and transformants were selected on Basta medium. In total, 83 9-month-old transgenic plants of Picea abies from six transformed sublines were analysed for continued tolerance to Basta. PCR analysis showed that the bar gene was present in all transformed plants but not in the control plants. Northern blot analysis showed… CONTINUE READING
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