Basophil counting with a new staining method using alcian blue.

@article{Gilbert1975BasophilCW,
  title={Basophil counting with a new staining method using alcian blue.},
  author={Harriet S. Gilbert and Leonard Ornstein},
  journal={Blood},
  year={1975},
  volume={46 2},
  pages={279-86}
}
Difficulties in obtaining reproducible and accurate enumeration of circulating basophils with existing techniques have hampered investigation of this infrequent cell population. A new basophil staining method is described that employs alcian blue dye for staining of heparin within basophils at low pH and in the presence of lanthanum ions. Basophil recognition is facilitated by reducing nonspecific nuclear staining. This objective is achieved because of the differences in stability of alcian… CONTINUE READING