Basophil Activation Test for Chronic Urticaria

  title={Basophil Activation Test for Chronic Urticaria},
  author={Salvatore Chirumbolo},
  journal={Annals of Laboratory Medicine},
  pages={499 - 500}
  • S. Chirumbolo
  • Published 24 June 2016
  • Biology
  • Annals of Laboratory Medicine
Dear Editor, Zehwan Kim et al, [1] presented a protocol to assay basophil activation by flow cytometry (FC) that included a phenotyping panel with CD123 and CD193 (CC chemokine receptor-3 [CCR3]). This method for the electronic capture of basophils in whole blood, never published before,raises some interesting questions. CCR3 (CD193), the eotaxin receptor, is commonly present in CD3-expressing T-cells [2,3] and it would be interesting to assess whetherthe basophil gating also included… 

Figures from this paper

Basophils priming in patients with chronic spontaneous urticaria
The results indicate a greater degree of basophils activation in patients with CSU in remission than in the control group, which might be useful for identification of patients with predominance of the autoimmune variant of CSU and typing patients responding (responders) and refractory (non- responders) to treatment with antihistamines.
Reply to the Letter to the Editor by Dr. Chirumbolo
  • D. Won
  • Biology, Medicine
    Annals of laboratory medicine
  • 2016
Gate 3 for purified basophils was the intersection area of the CD123bright and CCR3pos populations, and these two combined basophil identification markers may reciprocally help to exclude T lymphocytes (CD123neg) and monocytes (CCR3neg), even without additional exclusion markers.
Reply to “On the reliability of the CD123‐endowed basophil activation test (BAT) and its application in food allergy”
In response to the comments of Chirumbolo et al. concerning our recent paper1 , we note that this group has published comments with similar concerns, in reply to various papers over the past several


CCR3 for basophil activation test: a necessary but insufficient step
  • G. Monneret
  • Biology
    Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology
  • 2010
There is now accumulating evidence indicating that the basophil activation test (BAT), using either CD203c or CD63 as activation markers, has become a robust and reliable tool usable in clinical practice for the in vitro investigation of immediate allergy.
The use of IL‐3 in basophil activation tests is the real pitfall
The article tries to assess the use of basophil activation tests (BATs) based on CD203c upregulation as a reliable tool for allergy diagnosis and suggests how to use BATs that evaluate CD203C upregulation.
CCR3 as a single selection marker compared to CD123/HLADR to isolate basophils in flow cytometry: Some comments
This work suggested the use of eotaxin receptor CCR3, also known as CD193, as a single selection marker to separate basophils from other leukocytes in flow cytometry, though with some controversial issue.
Differential response of human basophil activation markers: a multi-parameter flow cytometry approach
Use of polychromatic flow cytometry allowed efficient basophil electronic purification and identification of different behaviors of the major activation markers, including CD203c as a more sensitive marker than CD63, in response to fMLP but not inresponse to anti-IgE and to calcium ionophore.
Basophil Markers for Identification and Activation in the Indirect Basophil Activation Test by Flow Cytometry for Diagnosis of Autoimmune Urticaria
The indirect basophil activation test using flow cytometry is a promising tool for autoimmune urticaria diagnosis and a combination of CD123 and CCR3 is recommended, while CD123 alone and CD63 alone on primed basophils may be used as an alternative.
Basophil Activation Test in Allergy: Time for an Update?
  • S. Chirumbolo
  • Biology
    International Archives of Allergy and Immunology
  • 2012
BAT is certainly a useful technique, also for isolated cases of hypersensitivity to various other compounds and drugs, and an update of its application is certainly an interesting topic to expand the debate on allergy diagnosis.
Serum‐induced basophil CD63 expression by means of a tricolour flow cytometric method for the in vitro diagnosis of chronic urticaria
A study to standardize the serum‐induced basophil activation assay by flow cytometry (FCM) using a new tricolour method, assessing the diagnostic performance of this test in discriminating between ASST+ and ASST− CU patients.
Autoantibodies to the high-affinity IgE receptor in chronic urticaria: how important are they?
  • J. Sheikh
  • Medicine, Biology
    Current opinion in allergy and clinical immunology
  • 2005
The recent evidence discussed in this review helps to clarify the role of autoantibodies in some cases of urticaria, but also points towards other non-autoimmune mechanisms that might be pathogenic.
Expression of a Functional Eotaxin (CC Chemokine Ligand 11) Receptor CCR3 by Human Dendritic Cells1 2 3
It is postulated that expression of CCR3 may underlie situations where both DCs and eosinophils accumulate in vivo, such as the lesions of patients with Langerhans cell granulomatosis.
Flow‐assisted allergy diagnosis: current applications and future perspectives
The technique proves valuable in the diagnosis of non‐IgE‐mediated (anaphylactoid) reactions such drug hypersensitivity and the detection of autoantibodies in certain forms of chronic urticaria and a viewpoint on how the field might evolve in the following years is provided.