Basidiospore allergens: determination of optimal extraction methods

@article{Liengswangwong1987BasidiosporeAD,
  title={Basidiospore allergens: determination of optimal extraction methods},
  author={V Liengswangwong and John E. Salvaggio and F. L. Lyon and Samuel B. Lehrer},
  journal={Clinical \& Experimental Allergy},
  year={1987},
  volume={17}
}
Five methods were tested by RAST for allergen extraction from seven basidiospore species Armillaria tabescens, Chlorophyllum molybdites, Coprinus quadrifidus, Pleurotus ostreatus, Calvatia cyathiformis, Pisolithus tinctorius, and Scleroderma sp. With each basidiospore type, extracted allergen activity varied according to the method employed. In general, defatting of spores with ethyl ether, followed by homogenization in 0.125 m NH4HCO3 buffer, resulted in greatest allergen yield. In order to… 

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