Basidiospore allergens: determination of optimal extraction methods

  title={Basidiospore allergens: determination of optimal extraction methods},
  author={V Liengswangwong and John E. Salvaggio and F. L. Lyon and Samuel B. Lehrer},
  journal={Clinical \& Experimental Allergy},
Five methods were tested by RAST for allergen extraction from seven basidiospore species Armillaria tabescens, Chlorophyllum molybdites, Coprinus quadrifidus, Pleurotus ostreatus, Calvatia cyathiformis, Pisolithus tinctorius, and Scleroderma sp. With each basidiospore type, extracted allergen activity varied according to the method employed. In general, defatting of spores with ethyl ether, followed by homogenization in 0.125 m NH4HCO3 buffer, resulted in greatest allergen yield. In order to… 


Although Calvatia species demonstrate a number of similarities, there are distinctive structural and allergenic properties that indicate that C. craniiformis and C. rubroflava are potentially important aeroallergens.

Myxomycete (slime mold) spores: unrecognized aeroallergens?

  • M. Lierl
  • Medicine
    Annals of allergy, asthma & immunology : official publication of the American College of Allergy, Asthma, & Immunology
  • 2013

Allergenicity of airborne basidiospores and ascospores: need for further studies

Evidence is provided that the role of basidiomycetes and meiotic forms of ascomycete deserves more attention with respect to their roles as potential aeroallergens.

Serological Reactivity and Identification of Immunoglobulin E-Binding Polypeptides of Ganoderma applanatum Crude Spore Cytoplasmic Extract in Puerto Rican Subjects

G. applanatum spores have allergenic components recognized by Puerto Rican individuals, which could eventually be considered as markers in cases of fungal allergy and be included in diagnostic allergen panels in Puerto Rico and tropical regions.

Prevalence of IgE reactivities in mold-allergic subjects to commercially available fungal enzymes.

The studies suggest that fungal enzymes may be useful source materials for the identification of fungal allergens and may also provide readily available source materials to produce improved diagnostic and therapeutic reagents.

Children with acute asthma: IgE antibody reactivities to fungi, house dust mites, cockroaches, and other indoor allergens.

Assessment of immunoglobulin E (IgE) antibody reactivity to fungi, basidiomycetes and fungi imperfecti, house dust mites, cockroaches, and other indoor allergens in young asthmatics with acute asthma exacerbation found a substantial increase in mite-specific positive RAST reactivity compared to younger children.

The Fungal Spore and Disease Initiation in Plants and Animals

This work presents a meta-analyses of the molecular Basis of Candida Pathogenicity and its relationships with Virulence in the Early Stages of Phaeohyphomycosis, and the role of cuticle-Degrading Enzymes in Fungal Pathogenesis in Insects.

The Fungal Spore

Surveys of fungal populations in the atmosphere have been performed continuously in many parts of the world and have provided a very long list of about 200,000 identified mold species.

Evaluation of the allergenicity of spore and mycelia extracts of Pisolithus tinctorius.

The importance of Pisolithus tinctorius, found as mycorrhiza of Eucalyptus spp, and this plant is used in reforestation in most countries, should be regarded as a possible cause of respiratory allergies, especially in occupationally exposed workers.



The significance of the spores of the Basidiomycetes (mushrooms and their allies) in bronchial asthma and allergic rhinitis.

It is demonstrated that patients with bronchial asthma have a higher incidence of immediate skin sensitivity by intradermal testing, whereas patients with allergic rhinitis have late phase sensitivity to these fungal spores.

The allergenic significance of certain fungi rarely reported as allergens.

The allergenic significance of seven different species of fungi was investigated and forty-four patients, representing 56% of the total number tested, demonstrated dermal reactivity toward one or more of the extracts.

Human sensitization to Ganoderma antigen.

Allergenicity and immunogenicity of Basidiomycetes.

Possible Role of Basidiospores as Air-borne Allergens

THE role of air-borne mould spores in asthma, especially Alternaria and Cladosporium, has been recognized as the result of the work of Feinberg1 and Durham2. These workers used mainly the gravity

Basidiomycete allergy: measurement of spore-specific IgE antibodies.

The Mushroom Hunter's Field Guide

"The Mushroom Hunter's Field Guide" is the guide that tells when, where, and how to find delicious edible mushrooms and how to avoid poisonous ones. Beginners as well as experts will be able to