Basidiomycete cryopreservation on perlite: evaluation of a new method.

@article{Homolka2006BasidiomyceteCO,
  title={Basidiomycete cryopreservation on perlite: evaluation of a new method.},
  author={Ladislav Homolka and Ludmila Lis{\'a} and Franti{\vs}ek Nerud},
  journal={Cryobiology},
  year={2006},
  volume={52 3},
  pages={446-53}
}
A new cryopreservation method using perlite as a carrier was evaluated on a large set of mycelial cultures of basidiomycetes. The viability and some other characteristics--growth, macro- and micromorphology, and laccase production--of 442 strains were tested after 48-h and then after 3-year storage in liquid nitrogen using a perlite protocol (PP). All (100%) of them survived successfully both 48-h storage and 3-year storage in liquid nitrogen without noticeable growth and morphological changes… CONTINUE READING

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