Basic fibroblast growth factor induces a transformed phenotype in normal human melanocytes

@article{Nesbit1999BasicFG,
  title={Basic fibroblast growth factor induces a transformed phenotype in normal human melanocytes},
  author={Mark Nesbit and Heike KE Nesbit and Jean Bennett and Thomas Andl and Mei-Yu Hsu and Emma Dejesus and Michelle McBrian and A. Ratan Gupta and Stephen L. Eck and Meenhard Herlyn},
  journal={Oncogene},
  year={1999},
  volume={18},
  pages={6469-6476}
}
Basic fibroblast growth factor (bFGF or FGF-2) is produced by nearly all melanomas in vitro and in vivo but not by normal melanocytes, which require exogenous bFGF for growth. In this study, we transduced normal human melanocytes to overexpress two forms of bFGF: (bFGF-Long and bFGF-Short) using replication-deficient adenovirus 5 vectors. bFGF-Long induced the 17.8, 22.5, 23.1 and 24.2 kDa forms of bFGF, whereas bFGF-Short induced only the 17.8 kDa mature form. Growth of cultured melanocytes… CONTINUE READING