Basement membrane-dependent modification of phenotype and gene expression in human retinal pigment epithelial ARPE-19 cells.

Abstract

PURPOSE To use porcine lens capsule (PLC) as basement membrane for ARPE-19 cells and to characterize its effects on cell differentiation and gene expression. METHODS Postconfluent cultures of ARPE-19 cells were established on either porous polyester filters or PLC membranes and characterized by electron microscopy, immunocytochemistry, and transepithelial… (More)

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