Base-type-selective high-resolution 13C edited NOESY for sequential assignment of large RNAs.

Abstract

Extensive spectral overlap presents a major problem for the NMR study of large RNAs. Here we present NMR techniques for resolution enhancement and spectral simplification of fully 13C labelled RNA. High-resolution 1H-13C correlation spectra are obtained by combining TROSY-type experiments with multiple-band-selective homonuclear 13C decoupling. An… (More)

Topics

  • Presentations referencing similar topics