Base sequence discrimination by zinc-finger DNA-binding domains

  title={Base sequence discrimination by zinc-finger DNA-binding domains},
  author={Jeannette Nardelli and Toby J. Gibson and Christine Vesque and Patrick Charnay},
ZINC fingers1,2 constitute important eukaryotic DNA-binding domains, being present in many transcription factors3–5. The Cys2/His2zinc-finger class has conserved motifs of 28–30 amino acids which are usually present as tandem repeats1,2. The structure of a Cys2/His2 zinc finger has been determined by nuclear magnetic resonance6, but details of its interaction with DNA were not established. Here we identify amino acids governing DNA-binding specificity using in vitro directed mutagenesis guided… CONTINUE READING

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