Base pairing properties of 8-oxo-7,8-dihydroadenosine in cDNA synthesis by reverse transcriptases.

Abstract

Incorporation of nucleotides opposite 8-oxo-7,8-dihydroadenosine (8-oxoA) in oligonucleotides with dNTPs by three reverse transcriptases (AMV-, MMRV-, RAV2-RT) in cDNA synthesis was studied. Guanine as well as thymine was incorporated preferentially by all reverse transcriptases. In the melting temperature experiment, 8-oxoA and 8-oxoA-Me formed base pairs… (More)

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Cite this paper

@article{Kim2002BasePP, title={Base pairing properties of 8-oxo-7,8-dihydroadenosine in cDNA synthesis by reverse transcriptases.}, author={Sang Kook Kim and Ji Young Kim and Ae Kyeong Baek and Byung Jo Moon}, journal={Bioorganic & medicinal chemistry letters}, year={2002}, volume={12 15}, pages={1977-80} }