Base pair hydrogen bonds are essential for proofreading selectivity by the human mitochondrial DNA polymerase.

@article{Lee2008BasePH,
  title={Base pair hydrogen bonds are essential for proofreading selectivity by the human mitochondrial DNA polymerase.},
  author={Harold R Lee and Sandra A Helquist and Eric T. Kool and K A Johnson},
  journal={The Journal of biological chemistry},
  year={2008},
  volume={283 21},
  pages={14411-6}
}
We have characterized the role of Watson-Crick hydrogen bonding in the 3'-terminal base pair on the 3'-5' exonuclease activity of the human mitochondrial DNA polymerase. Nonpolar nucleoside analogs of thymidine (dF) and deoxyadenosine (dQ) were used to eliminate hydrogen bonds while maintaining base pair size and shape. Exonuclease reactions were examined using pre-steady state kinetic methods. The time dependence of removal of natural nucleotides from the primer terminus paired opposite the… CONTINUE READING