Base flipping in V(D)J recombination: insights into the mechanism of hairpin formation, the 12/23 rule, and the coordination of double-strand breaks.

@article{Bischerour2009BaseFI,
  title={Base flipping in V(D)J recombination: insights into the mechanism of hairpin formation, the 12/23 rule, and the coordination of double-strand breaks.},
  author={Julien Bischerour and Catherine Lu and David B Roth and Ronald Chalmers},
  journal={Molecular and cellular biology},
  year={2009},
  volume={29 21},
  pages={5889-99}
}
Tn5 transposase cleaves the transposon end using a hairpin intermediate on the transposon end. This involves a flipped base that is stacked against a tryptophan residue in the protein. However, many other members of the cut-and-paste transposase family, including the RAG1 protein, produce a hairpin on the flanking DNA. We have investigated the reversed… CONTINUE READING