Base damage immediately upstream from double-strand break ends is a more severe impediment to nonhomologous end joining than blocked 3'-termini.

@article{Datta2010BaseDI,
  title={Base damage immediately upstream from double-strand break ends is a more severe impediment to nonhomologous end joining than blocked 3'-termini.},
  author={Kamal Datta and Shubhadeep Purkayastha and Ronald D. Neumann and Elzbieta Pastwa and Thomas A. Winters},
  journal={Radiation research},
  year={2010},
  volume={175 1},
  pages={
          97-112
        }
}
Radiation-induced DNA double-strand breaks (DSBs) are critical cytotoxic lesions that are typically repaired by nonhomologous end joining (NHEJ) in human cells. Our previous work indicated that the highly cytotoxic DSBs formed by (125)I decay possess base damage clustered within 8 to 10 bases of the break and 3'-phosphate (P) and 3'-OH ends. This study examined the effect of such structures on NHEJ in in vitro assays employing either (125)I decay-induced DSB linearized plasmid DNA or… CONTINUE READING

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