Barnase and barstar: two small proteins to fold and fit together.

@article{Hartley1989BarnaseAB,
  title={Barnase and barstar: two small proteins to fold and fit together.},
  author={Robert W. Hartley},
  journal={Trends in biochemical sciences},
  year={1989},
  volume={14 11},
  pages={450-4}
}
Barnase and barstar are the extracellular ribonuclease and its intracellular inhibitor produced by Bacillus amyloliquefaciens. Both are small single-chain proteins and thus are suitable for application to the study of how a protein's sequence directs its fold. Barnase has neither disulfide bonds nor non-peptide components and unfolds reversibly in what closely approximates a two-state reaction. The genes for both these proteins have been cloned in E. coli. Expression of barstar is necessary to… CONTINUE READING