Barley anther culture: assessment of carbohydrate effects on embryo yield, green plant production and differential plastid development in relation with albinism.

@article{Wojnarowiez2004BarleyAC,
  title={Barley anther culture: assessment of carbohydrate effects on embryo yield, green plant production and differential plastid development in relation with albinism.},
  author={Genevi{\`e}ve Wojnarowiez and St{\'e}phane Caredda and Pierre M. Devaux and Rajbir S. Sangwan and Christophe Cl{\'e}ment},
  journal={Journal of plant physiology},
  year={2004},
  volume={161 6},
  pages={747-55}
}
Sugars and polyols were tested at different steps of anther culture in barley (Hordeum vulgare L.) to elucidate their influence on both the overall yield of androgenesis and the structure of plastids in relation to albinism. During the pretreatment period, the osmotic regulation in the medium was beneficial to microspore embryogenesis regardless of the type and concentration of the tested osmoticum. The use of mannitol (300 mOsm/kg), sorbitol (180 mOsm/kg), PEG (240 mOsm/kg) and sucrose (180… CONTINUE READING
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