Barcoded DNA nanostructures for the multiplexed profiling of subcellular protein distribution

@article{Sundah2019BarcodedDN,
  title={Barcoded DNA nanostructures for the multiplexed profiling of subcellular protein distribution},
  author={Noah R. Sundah and Nicholas R. Y. Ho and Geok Soon Lim and Auginia Natalia and Xianguang Ding and Yu Liu and Ju Ee Seet and Ching Wan Chan and Tze Ping Loh and Huilin Shao},
  journal={Nature Biomedical Engineering},
  year={2019},
  pages={1-11}
}
Massively parallel DNA sequencing is established, yet high-throughput protein profiling remains challenging. Here, we report a barcoding approach that leverages the combinatorial sequence content and the configurational programmability of DNA nanostructures for high-throughput multiplexed profiling of the subcellular expression and distribution of proteins in whole cells. The barcodes are formed by in situ hybridization of tetrahedral DNA nanostructures and short DNA sequences conjugated with… 

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