Balance between MAT2A intron detention and splicing is determined cotranscriptionally.

@article{Pendleton2018BalanceBM,
  title={Balance between MAT2A intron detention and splicing is determined cotranscriptionally.},
  author={Kathryn E. Pendleton and Sung-Kyun Park and Olga V. Hunter and Stefan M. Bresson and Nicholas K Conrad},
  journal={RNA},
  year={2018},
  volume={24 6},
  pages={
          778-786
        }
}
Transcriptome analysis of human cells has revealed that intron retention controls the expression of a large number of genes with diverse cellular functions. Detained introns (DI) constitute a subgroup of transcripts with retained introns that are not exported to the cytoplasm but instead remain in the nucleus. Previous studies reported that the splicing of DIs in the CLK1 transcript is post-transcriptionally induced to produce mature mRNA in the absence of new transcription. Thus, CLK1-DI… Expand
SAM homeostasis is regulated by CFIm-mediated splicing of MAT2A
S-adenosylmethionine (SAM) is the methyl donor for nearly all cellular methylation events. Cells regulate intracellular SAM levels through intron detention of the MAT2A RNA, which encodes only SAMExpand
SAM homeostasis is regulated by CFIm-mediated splicing of MAT2A
S-adenosylmethionine (SAM) is the methyl donor for nearly all cellular methylation events. Cells regulate intracellular SAM levels through intron detention of MAT2A, the only SAM synthetase expressedExpand
O-GlcNAc regulates gene expression by controlling detained intron splicing
TLDR
It is shown that a ubiquitous post-translational modification called O-GlcNAc, which is thought to integrate signaling pathways as nutrient conditions fluctuate, controls detained intron splicing to tune system-wide gene expression. Expand
O-GlcNAc regulates gene expression by controlling detained intron splicing
TLDR
It is demonstrated that a ubiquitous post-translational modification called O-GlcNAc controls detained intron splicing to tune system-wide gene expression, providing a means to couple nutrient conditions to the cell's transcriptional regime. Expand
Nuclear mechanisms of gene expression control: pre-mRNA splicing as a life or death decision.
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The possibility that proximity of RNA to nuclear speckles - biomolecular condensates that are highly enriched in splicing factors and other RNA binding proteins - is associated with choices ranging from efficient co-transcribed splicing, export and stability to regulated post-transcriptional splicing and possible vulnerability to decay is discussed. Expand
An important class of intron retention events in human erythroblasts is regulated by cryptic exons proposed to function as splicing decoys
TLDR
RNA-seq analysis of nonsense-mediated decay (NMD)-inhibited cells revealed previously undescribed splice junctions that connect constitutive exons 4 and 5 to highly conserved cryptic cassette exons within the intron, suggesting developmental regulation of decoy function underlies a major component of the erythroblast IR program. Expand
An important class of intron retention events in human erythroblasts is regulated by cryptic exons proposed to function as splicing decoys.
TLDR
RNA-seq analysis of nonsense-mediated decay-inhibited cells revealed previously undescribed splice junctions that connect constitutive exons 4 and 5 to highly conserved cryptic cassette exons within the intron, and it is proposed that these exons function as decoys that engage the introns-terminal splice sites, thereby blocking cross-intron interactions required for excision. Expand
Antisense targeting of decoy exons can reduce intron retention and increase protein expression in human erythroblasts.
TLDR
Results show that modulating decoy exon function can dramatically alter IR, and suggest that dynamic regulation of decoyExons could be a mechanism to fine tune gene expression post-transcriptionally in many cell types. Expand
Antisense targeting of decoy exons can reduce intron retention and increase protein expression in human erythroblasts
TLDR
Results show that modulating decoy exon function can dramatically alter IR, and suggest that dynamic regulation of decoyExons could be a mechanism to fine tune gene expression post-transcriptionally in many cell types. Expand
METTL16, Methyltransferase-Like Protein 16: Current Insights into Structure and Function
  • A. Ruszkowska
  • Medicine
  • International journal of molecular sciences
  • 2021
TLDR
The interaction between METTL16 and oncogenic lncRNA MALAT1 indicates the existence ofMETTL16 features specifically recognizing RNA triple helices, and the number of known human m6A methyltransferases has grown from one to five during the last five years. Expand
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