Bag-1M accelerates nucleotide release for human Hsc70 and Hsp70 and can act concentration-dependent as positive and negative cofactor.

@article{Gassler2001Bag1MAN,
  title={Bag-1M accelerates nucleotide release for human Hsc70 and Hsp70 and can act concentration-dependent as positive and negative cofactor.},
  author={C S Gassler and Thomas Wiederkehr and Dirk Brehmer and Bernd Bukau and Matthias P. Mayer},
  journal={The Journal of biological chemistry},
  year={2001},
  volume={276 35},
  pages={32538-44}
}
The cytosol of mammalian cells contains several Hsp70 chaperones and an arsenal of cochaperones, including the anti-apoptotic Bag-1M protein, which regulate the activities of Hsp70s by controlling their ATPase cycles. To elucidate the regulatory function of Bag-1M, we determined its influence on nucleotide exchange, substrate release, ATPase rate, and chaperone activity of the housekeeping Hsc70 and stress-inducible Hsp70 homologs of humans. Bag-1M and a C-terminal fragment of it are potent… CONTINUE READING