Baculovirus-expressed constructs induce immunoglobulin G that recognizes VAR2CSA on Plasmodium falciparum-infected erythrocytes.

Abstract

We raised specific antisera against recombinant VAR2CSA domains produced in Escherichia coli and in insect cells. All were reactive in enzyme-linked immunosorbent assay, but only insect cell-derived constructs induced immunoglobulin G (IgG) that was reactive with native VAR2CSA on the surface of infected erythrocytes. Our data show that five of the six VAR2CSA Duffy-binding-like domains are surface exposed and that induction of surface-reactive VAR2CSA-specific IgG depends critically upon antigen conformation. These findings have implications for the development of vaccines against pregnancy-associated Plasmodium falciparum malaria.

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@article{Barfod2006BaculovirusexpressedCI, title={Baculovirus-expressed constructs induce immunoglobulin G that recognizes VAR2CSA on Plasmodium falciparum-infected erythrocytes.}, author={Lea Barfod and Morten Agertoug Nielsen and Louise A. Turner and Madeleine A Dahlb{\"a}ck and Anja T R Jensen and Lars Hviid and Thor G. Theander and Ali Salanti}, journal={Infection and immunity}, year={2006}, volume={74 7}, pages={4357-60} }