Bactofection of mammalian cells by Listeria monocytogenes: improvement and mechanism of DNA delivery

@article{Pilgrim2003BactofectionOM,
  title={Bactofection of mammalian cells by Listeria monocytogenes: improvement and mechanism of DNA delivery},
  author={S. A. L. Pilgrim and Jochen Stritzker and Cor D. Schoen and Annette Kolb-M{\"a}urer and Gernot Geginat and Martin J Loessner and Ivaylo Gentschev and W. Goebel},
  journal={Gene Therapy},
  year={2003},
  volume={10},
  pages={2036-2045}
}
Bacteria-mediated transfer of plasmid DNA into mammalian cells (bactofection) is a potent approach to express plasmid-encoded heterologous proteins (protein antigens, toxins or enzymes) in a large set of different cell types including phagocytic and nonphagocytic mammalian cells. Previously, we have described a Listeria monocytogenes-mediated DNA delivery system, which releases plasmid DNA directly into the cytosol of mammalian cells by partial self-destruction of the carrier bacteria. Here we… CONTINUE READING
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