Bacteroides gingivalis fimbriae stimulate production of thymocyte-activating factor by human gingival fibroblasts

  title={Bacteroides gingivalis fimbriae stimulate production of thymocyte-activating factor by human gingival fibroblasts},
  author={Shigemasa Hanazawa and Kimiharu Hirose and Yoshihiro Ohmori and Shigeru Amano and Shigeo Kitano},
  journal={Infection and Immunity},
  pages={272 - 274}
In a previous report (Y. Ohmori, S. Hanazawa, S. Amano, T. Miyoshi, K. Hirose, and S. Kitano, infect. Immun. 55:947-954, 1987), we showed that human gingival fibroblasts spontaneously produce thymocyte-activating factor (FTAF), which stimulates mitogen-induced thymocyte proliferation. In the present study, we examined the effect of Bacteroides gingivalis fimbriae on FTAF production by the cells, because the fimbriae may be involved in attachment of the organism to periodontal tissues. We show… 
Porphyromonas gingivalis fimbria-stimulated bone resorption in vitro is inhibited by a tyrosine kinase inhibitor
Genistein, a potent inhibitor of tyrosine kinase, markedly inhibited bone resorption stimulated by the fimbriae, and inhibited induction of several tyrosinesine-phosphorylated proteins in thefimbria-treated calvarial bone cells from mouse embryos.
Adhesion of Porphyromonas gingivalis fimbriae to human gingival cell line Ca9-22.
The data on trypsin treatment suggest that elevated levels of protease in the gingival sulcus, such as can occur with poor oral hygiene and gingivitis, may expose adhesion molecules on theGingival cell surface, thereby promoting the attachment of P. gedivalis fimbriae.
Inductive effect of Porphyromonas gingivalis fimbriae on differentiation of human monocytic tumor cell line U937.
Results demonstrate that P. gingivalis fimbriae are a potent inducer of the differentiation of the monocyte/macrophage tumor cell line U937, most probably via cyclic nucleotide-independent protein kinase C.
Inducing effect of periodontopathic bacteria on interleukin-1 production by mouse peritoneal macrophages.
Sonicated extracts from Bacteroides gingivalis. Fusobacterium nucleatum, Actinobacillus actinomycetemcomitans, and Actinomyces viscosus were found to strongly induce IL-1 production by mouse
Direct and indirect effects of Porphyromonas gingivalis lipopolysaccharide on interleukin-6 production by human gingival fibroblasts.
The results suggest that, although gingival fibroblasts may be involved in the pathogenesis of chronic periodontal disease by the production of cytokines, such a role may not result from a direct stimulation by periodontopathic bacteria, and is more likely to be mediated indirectly by IL-1 produced by infiltrating inflammatory cells.
Bacteroides (Porphyromonas) gingivalis fimbriae activate mouse peritoneal macrophages and induce gene expression and production of interleukin-1
Observations suggest that B. gingivalis fimbriae may be involved in the pathogenesis of adult periodontal disease via triggering of IL-1 production by monocytes/macrophage activation of peritoneal macrophages inperiodontal diseases.
Fimbriae from Porphyromonas gingivalis Induce Chemiluminescence Response of Macrophages in a Different Manner to Its Lipopolysaccharide
Observations suggest that P. gingivalis fimbriae may be involved in the pathogenesis of adult periodontal disease via the triggering of reactive oxygen intermediates by macrophages in the disease in a different manner to its LPS.
Porphyromonas gingivalis fimbriae and their synthetic peptides induce proinflammatory cytokines in human peripheral blood monocyte cultures.
Porphyromonas gingivalis fimbriae as well as synthetic peptides that mimic the fimbrial subunit protein, which includes the amino acid sequence XLTXXLTXXNXX, induced high production of proinflammatory cytokines in human peripheral blood monocyte/macrophage cultures.
Porphyromonas gingivalis lipopolysaccharide modulates the responsiveness of human periodontal ligament fibroblasts to platelet-derived growth factor.
Results indicate that P. gingivalis LPS stimulate cellular proliferation and responsiveness to platelet-derived growth factor (PDGF)-BB of cultured HPLF, mediated by PDGF-BB binding, followed by increased synthesis of the receptor protein.
Porphyromonas gingivalis fimbria-stimulated bone resorption is inhibited through binding of the fimbriae to fibronectin
This present study demonstrates with mouse embryonic calvarial cells that P. gingivalis fimbria-stimulated bone resorption is inhibited by human fibronectin.


Spontaneous production of thymocyte-activating factor by human gingival fibroblasts and its autoregulatory effect on their proliferation
The observations suggest that gingival fibroblasts play a significant role in regulation of cell growth of lymphocytes and in their own growth under physiological conditions and in pathological states in periodontal connective tissue.
Increased thymocyte-activating factor in human gingival fluid during gingival inflammation
It is suggested that human gingival fluid contains epidermal thymocyte-activating factor or interleukin 1 or both, which may amplify the inflammatory response in periodontal tissues.
Murine epidermal cell-derived thymocyte-activating factor resembles murine interleukin 1.
Observations continue to support the view that ETAF may be identical or closely related to IL 1, and suggest that "injurious" stimuli that prolong the G1 phase of the cell cycle factor ETAf production appear to be a mitogenic signal for fibroblasts.
Production of prostaglandin E and an interleukin-1 like factor by cultured astrocytes and C6 glioma cells.
Astrocytes may interact with the immune system by elaborating nonspecific factors that modulate lymphocyte proliferation, which may be important in the generation of specific immune responses in the brain, which is considered to be an immunologically privileged organ.
Purification and characterization of a novel type of fimbriae from the oral anaerobe Bacteroides gingivalis
Fimbriae and their constituent protein (fimbrilin) were purified to homogeneity from the bacterial wash fluid and cell lysate fraction, respectively, of Bacteroides gingivalis 381, and it has been inferred on the basis of circumstantial evidence that fimbRIae are correlated to hemagglutinating activity of the organism.
Functional role of interleukin 1 in periodontal disease: induction of interleukin 1 production by Bacteroides gingivalis lipopolysaccharide in peritoneal macrophages from C3H/HeN and C3H/HeJ mice
It is suggested that B-LPS-induced IL-1 may play a significant role in the pathogenesis of adult periodontal disease.
Humoral antibody response against Bacteroides gingivalis-specific antigen recognized by monoclonal antibody in adult periodontal patients
Results indicate that the purified antigen was not cross-reactive with rat polyclonal antibodies to each of several black-pigmented Bacteroides species, Fusobacterium nucleatum, Eikenella corrodens, and Actinobacillus actinomycetemcomitans.
Serum antibodies to oral Bacteroides asaccharolyticus (Bacteroides gingivalis): relationship to age and periondontal disease
Serum samples from patients with various periodontal diseases indicated that antibodies to oral B. asaccharolyticus occur at low levels in most normal children and adults and that the rise in titer of the specific antibodies of each major class of immunoglobulins parallels the ontogenic change in serum levels of that isotype.
Fimbriae from the oral anaerobe Bacteroides gingivalis: physical, chemical, and immunological properties
Examination of antibodies against fimbriae and sodium dodecyl sulfate-denatured fimbrilin by enzyme-linked immunosorbent assay reinforced a previous notion that different sets of antigenic determinants seemed to be exposed on their surfaces.
Importance of black-pigmented Bacteroides in human periodontal disease, p. 27-45
  • Host - parasite interactions in periodontal disease
  • 1982