Bacteroides forsythus in primary endodontic infections as detected by nested PCR.

@article{Siqueira2003BacteroidesFI,
  title={Bacteroides forsythus in primary endodontic infections as detected by nested PCR.},
  author={Jos{\'e} F Siqueira and Isabela das Neves R{\^o}ças},
  journal={Journal of endodontics},
  year={2003},
  volume={29 6},
  pages={390-3}
}
The purpose of this study was to investigate the prevalence of Bacteroides forsythus in primary endodontic infections using a species-specific nested polymerase chain reaction assay. Samples were collected from 50 teeth having carious lesions, necrotic pulps, and different forms of periradicular diseases. DNA extracted from the samples was initially amplified using universal 16S rDNA primers. A second round of amplification used the first polymerase chain reaction products to detect a specific… CONTINUE READING

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