Bacteroides (Porphyromonas) gingivalis fimbriae activate mouse peritoneal macrophages and induce gene expression and production of interleukin-1

  title={Bacteroides (Porphyromonas) gingivalis fimbriae activate mouse peritoneal macrophages and induce gene expression and production of interleukin-1},
  author={Shigemasa Hanazawa and Yukio Murakami and Kimiharu Hirose and Shigeru Amano and Yoshihiro Ohmori and Hiroshi Higuchi and Shigeo Kitano},
  journal={Infection and Immunity},
  pages={1972 - 1977}
The purpose of this study was to examine whether Bacteroides (Porphyromonas) gingivalis fimbriae, an important structure involved in attachment of the bacteria to periodontal tissues, activate macrophages and subsequently induce gene expression and production of interleukin-1 (IL-1) in the cells. The fimbriae increased glucose consumption and lysozyme activity in BALB/c macrophages, both criteria of macrophage activation of peritoneal macrophages, in a dose-dependent fashion. A marked increase… 

Porphyromonas gulae 41-kDa fimbriae induced osteoclast differentiation and cytokine production

Osteoclast differentiation was significantly enhanced by treatment with the 41-kDa fimbrial protein in a dose-dependent manner and the bone loss level in rats infected with P. gulae was significantly higher than that of the sham-infected rats.

Porphyromonas gingivalis fimbriae stimulate bone resorption in vitro

The present data demonstrate that P. gingivalis fimbriae stimulate bone resorption in vitro via the generation of an inflammatory cytokine(s) and these cytokines were markedly expressed in the fimbria-treated calvarial bone cells.

Porphyromonas gingivalis fimbriae induce a 68-kilodalton phosphorylated protein in macrophages

The fimbriae induced a 68-kDa phosphorylated protein (pp68) in a dose-dependent manner in mouse peritoneal macrophages, and it was observed that gene expression of the fimbria-induced neutrophil chemoattractant KC was inhibited markedly by staurosporine.

Differential cytokine induction by two types of Porphyromonas gingivalis.

Although other factors may also be involved, the sustained cytokine response induced by type II P. gingivalis may play an important role in enhanced periodontal tissue inflammation and destruction.

CpG Motifs in Porphyromonas gingivalis DNA Stimulate Interleukin-6 Expression in Human Gingival Fibroblasts

It is suggested here that Porphyromonas gingivalis DNA may function as a virulence factor in periodontal disease through expression of inflammatory cytokine through stimulation of transcriptional factor NF-κB.

Inductive effect of Porphyromonas gingivalis fimbriae on differentiation of human monocytic tumor cell line U937.

Results demonstrate that P. gingivalis fimbriae are a potent inducer of the differentiation of the monocyte/macrophage tumor cell line U937, most probably via cyclic nucleotide-independent protein kinase C.

Lipid A-associated proteins from periodontopathogenic bacteria induce interleukin-6 production by human gingival fibroblasts and monocytes.

The results show that bacterial cell wall components other than LPS can induce IL-6 release from cells of the periodontium in vitro, and the production of such potent immunomodulatory agents in vivo may contribute to the connective tissue breakdown characteristic of chronic periodontitis.

Cytokine production induced by a 67-kDa fimbrial protein from Porphyromonas gingivalis.

The results suggest that P. gingivalis 67-kDa fimbriae may play a part in the inflammatory response during the development of periodontal diseases.



Functional role of interleukin 1 in periodontal disease: induction of interleukin 1 production by Bacteroides gingivalis lipopolysaccharide in peritoneal macrophages from C3H/HeN and C3H/HeJ mice

It is suggested that B-LPS-induced IL-1 may play a significant role in the pathogenesis of adult periodontal disease.

Production of Interleukin-1 and Tumor Necrosis Factor by Human Peripheral Monocytes Activated by Periodontal Bacteria and Extracted Lipopolysaccharides

Results indicate that monocytes are activated by free LPS or LPS bound to Gram-negative pathogenicperiodontal bacteria to produce monokines which may contribute to the destruction of periodontal bone.

Bacteroides gingivalis fimbriae stimulate production of thymocyte-activating factor by human gingival fibroblasts

It is shown here that the fimbriae bind to the cells, which may subsequently lead to the stimulation of FTAF production by the cells.

Lipopolysaccharide-induced gene expression in murine peritoneal macrophages is selectively suppressed by agents that elevate intracellular cAMP.

Exchange of genes encoding inducible early proteins and inflammatory monokines are selectively regulated by elevation of intracellular cAMP, indicating that effects may be pleiotropic in nature involving multiple molecular mechanisms.

The effect of interleukin 1 beta on proteoglycans synthesized by human gingival fibroblasts in vitro.

  • P. Bartold
  • Biology, Medicine
    Connective tissue research
  • 1988
IL-1 beta can modulate extracellular matrix synthesis by human gingival fibroblasts and may therefore be partially responsible for the early events of healing following inflammatory episodes.

An Epstein-Barr virus-transformed B cell line produces autoregulatory interleukin-1 that regulates bone remodeling.

Cloning and expression of murine interleukin-1 cDNA in Escherichia coli

The cloning, sequence analysis and expression of murine IL-1 cDNA in Escherichia coli reveals a polypeptide precursor of 270 amino acids that may play a major role in the initiation and amplification of immune and inflammatory responses through its action on these diverse cell populations.

Spontaneous production of thymocyte-activating factor by human gingival fibroblasts and its autoregulatory effect on their proliferation

The observations suggest that gingival fibroblasts play a significant role in regulation of cell growth of lymphocytes and in their own growth under physiological conditions and in pathological states in periodontal connective tissue.

Interleukin-1 has independent effects on deoxyribonucleic acid and collagen synthesis in cultures of rat calvariae.

Indomethacin blocked the stimulatory effect on CDP and NCP labeling, suggesting a prostaglandin-mediated effect, but did not change the IL-1 effect on DNA synthesis, but exposure of the calvariae to highIL-1 doses or to IL- 1 for prolonged periods of time results in an inhibition of collagen synthesis.