Bacteriorhodopsin: partial sequence of mRNA provides amino acid sequence in the precursor region.

@article{Chang1981BacteriorhodopsinPS,
  title={Bacteriorhodopsin: partial sequence of mRNA provides amino acid sequence in the precursor region.},
  author={Simon H. Chang and Alokes Majumdar and Robert L. Dunn and O Makabe and Uttam L. RajBhandary and Har Gobind Khorana and Eiko Ohtsuka and Teruo Tanaka and Y. Taniyama and Morio Ikehara},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1981},
  volume={78 6},
  pages={3398-402}
}
mRNA for bacteriorhodopsin from Halobacterium halobium has been partially purified. By using this mRNA as template in the presence of reverse transcriptase RNA-dependent DNA nucleotidyltransferase and a 5'-[32P] synthetic oligodeoxyribonucleotide corresponding to amino acids 9-12 of bacteriorhodopsin as primer, we have isolated the major 5'-[32P]cDNA… CONTINUE READING