Bacteriophage phi 6 envelope elucidated by chemical cross-linking, immunodetection, and cryoelectron microscopy.

@article{Kenney1992BacteriophageP6,
  title={Bacteriophage phi 6 envelope elucidated by chemical cross-linking, immunodetection, and cryoelectron microscopy.},
  author={James M. Kenney and Jarkko Hantula and Stephen D. Fuller and Leonard Mindich and P{\"a}ivi M Ojala and Dennis Henry Bamford},
  journal={Virology},
  year={1992},
  volume={190 2},
  pages={
          635-44
        }
}
Bacteriophage phi 6 is an enveloped dsRNA virus which infects the plant pathogenic Pseudomonas syringae bacterium. Using low dose cryoelectron microscopy we show that the nucleocapsid, spikeless virion, and intact virion have radii of 29, 35, and 43 nm, respectively. Thus, the membrane is 6 nm thick and the surface spikes of the receptor binding protein P3 extend 8 nm from the membrane surface. Cross-linking, immunological, and complementation evidence suggest that the spikes are formed of… CONTINUE READING
Highly Cited
This paper has 40 citations. REVIEW CITATIONS

From This Paper

Topics from this paper.

Citations

Publications citing this paper.
Showing 1-10 of 16 extracted citations

Bacteriophage φ6--structure investigated by fluorescence Stokes shift spectroscopy.

Photochemistry and photobiology • 2012
View 5 Excerpts
Highly Influenced