Bacteriophage phi 1 as a gene-cloning vector in Bacillus subtilis.

Abstract

We attempted to use Bacillus subtilis phage phi 1 as a gene-cloning vector since the phi 1 genome was found to have few cleavage sites upon digestion with several kinds of restriction endonucleases. A phi 1 stock supplied by J. Ito (University of Arizona, Tucson, USA) consisted of two phages, phi 1E1 and phi 1E2, having one and two EcoRI-cleavage sites in… (More)

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@article{Kawamura1980BacteriophageP1, title={Bacteriophage phi 1 as a gene-cloning vector in Bacillus subtilis.}, author={Fujio Kawamura and Hidetsugu Saito and Y Ikeda}, journal={Molecular & general genetics : MGG}, year={1980}, volume={180 2}, pages={259-66} }