Bacteriophage lambda vector for transducing a cDNA clone library into mammalian cells.

@article{Okayama1985BacteriophageLV,
  title={Bacteriophage lambda vector for transducing a cDNA clone library into mammalian cells.},
  author={Hiroto Okayama and Paul Berg},
  journal={Molecular and cellular biology},
  year={1985},
  volume={5 5},
  pages={
          1136-42
        }
}
We have developed a bacteriophage lambda vector (lambda NMT) that permits efficient transduction of mammalian cells with a cDNA clone library constructed with the pcD expression vector (H. Okayama and P. Berg, Mol. Cell. Biol. 3:280-289, 1983). The phage vector contains a bacterial gene (neo) fused to the simian virus 40 early-region promoter and RNA processing signals, providing a dominant-acting selectable marker for mammalian transformation. The phage DNA can accommodate pcD-cDNA… CONTINUE READING
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