Bacteriophage lambda N protein alone can induce transcription antitermination in vitro.

@article{Rees1996BacteriophageLN,
  title={Bacteriophage lambda N protein alone can induce transcription antitermination in vitro.},
  author={William A Rees and Stephen E Weitzel and Thomas Yager and Asis Das and Peter H. von Hippel},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1996},
  volume={93 1},
  pages={342-6}
}
Specific and processive antitermination by bacteriophage lambda N protein in vivo and in vitro requires the participation of a large number of Escherichia coli proteins (Nus factors), as well as an RNA hairpin (boxB) within the nut site of the nascent transcript. In this study we show that efficient, though nonprocessive, antitermination can be induced by large concentrations of N alone, even in the absence of a nut site. By adding back individual components of the system, we also show that N… CONTINUE READING