Bacteriophage-based vectors for site-specific insertion of DNA in the chromosome of Corynebacteria.

@article{Oram2007BacteriophagebasedVF,
  title={Bacteriophage-based vectors for site-specific insertion of DNA in the chromosome of Corynebacteria.},
  author={Mark Oram and Joelle E Woolston and Andrew D. Jacobson and Randall K. Holmes and Diana Marra Oram},
  journal={Gene},
  year={2007},
  volume={391 1-2},
  pages={53-62}
}
In Corynebacterium diphtheriae, diphtheria toxin is encoded by the tox gene of some temperate corynephages such as beta. beta-like corynephages are capable of inserting into the C. diphtheriae chromosome at two specific sites, attB1 and attB2. Transcription of the phage-encoded tox gene, and many chromosomally encoded genes, is regulated by the DtxR protein in response to Fe(2+) levels. Characterizing DtxR-dependent gene regulation is pivotal in understanding diphtheria pathogenesis and… CONTINUE READING