Bacteriophage T7 E promoter: identification and measurement of kinetics of association with Escherichia coli RNA polymerase.

Abstract

The initiation point for transcription from the Escherichia coli RNA polymerase E promoter on bacteriophage T7 has been determined to be at 36 835 base pairs (92.22 T7 units) from the left end of T7. The location was determined by RNA fingerprinting of a runoff transcription product. Kinetics of association for the E and the T7 A3 promoters were measured by… (More)

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