Bacteriophage T 7 Deoxyribonucleic Acid Replication in Vitro

@inproceedings{Richardson2002BacteriophageT7,
  title={Bacteriophage T 7 Deoxyribonucleic Acid Replication in Vitro},
  author={Charles C. Richardson},
  year={2002}
}
(CHAMBERLIN, M. (1974) J. Virol. 14, 509-516). In uitro, extracts of tsnC mutant E. coli infected with T7 phage are incapable of replicating duplex T7 DNA, although extracts of wild type E. coli infected with T7 phage support replication of T7 DNA. In addition, extracts of the infected tsnC mutant are deficient in T7 DNA polymerase activity. Extracts prepared from uninfected E. coli tsnC + cells restore the ability of the infected tsnC extracts to replicate duplex T7 DNA, and also restore… CONTINUE READING