Bacteriological follow-up of pulmonary tuberculosis treatment: a study with a simple colorimetric assay.

@article{Jamileh2004BacteriologicalFO,
  title={Bacteriological follow-up of pulmonary tuberculosis treatment: a study with a simple colorimetric assay.},
  author={Nourouzi Jamileh and Foroozan Mohammadi and Mehdi Mirsaedi and Abolhasan Zia Zarifi and Javad Tabatabee and Moslem Bahadori and Ali Akbar Velayati and Mohammad Reza Masjedi},
  journal={Microbes and infection},
  year={2004},
  volume={6 11},
  pages={
          972-6
        }
}
The viability of Mycobacterium tuberculosis (MTB) in serial sputum specimens from persistently smear positive patients was evaluated. The assay was based on oxidation-reduction of Alamar Blue and Malachite Green dyes that change their color in response to MTB growth. A total of 280 sputum specimens from 40 persistently smear positive TB patients and 40 sputa from non-tuberculosis patients were digested, decontaminated and examined microscopically. To check the MTB viability, the sediments from… CONTINUE READING
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