• Corpus ID: 88589192

Bacteriocins: Unique approaches for the characterization of peptide antimicrobials in lactic acid bacteria

  title={Bacteriocins: Unique approaches for the characterization of peptide antimicrobials in lactic acid bacteria},
  author={S. Macwana},
iii ACKNOWLEDGMENT I wish to express my sincere appreciation to my advisor, Dr. Peter Muriana for his supervision, optimism and guidance which has enabled me to be an independent researcher. My extended appreciation to my committee members, Dr. Gilliland, Dr. McGlynn and Dr. DeWitt for serving on my graduate committee. Thank You for your support, suggestion, time and encouragement. I would also like to thank my parents, John and Victoria Macwana, for their love, encouragement, understanding… 


Analysis of genes involved in biosynthesis of the lantibiotic subtilin
The results clearly proved that reading frames spaB and spaC are essential for subtilin biosynthesis whereas spaT mutants are probably deficient in subtil in transport.
Developing applications for lactococcal bacteriocins
Strains which produce these inhibitors can be exploited in the acceleration of cheese ripening by assisting the premature lysis of starter cultures, and there is also considerable potential for biomedical applications.
Production of class II bacteriocins by lactic acid bacteria; an example of biological warfare and communication
Although today a lot is known about LAB bacteriocins and the regulation of their production, several fundamental questions remain to be solved, including questions regarding mechanisms of immunity and resistance, as well as the molecular basis of target-cell specificity.
Bacteriocins: Biological tools for bio-preservation and shelf-life extension
Some of the advances made, made mainly with other lactococcal bacteriocins, in improving food safety, food quality and preventing food spoilage are discussed.
Biosynthesis of the Lantibiotic Mersacidin: Organization of a Type B Lantibiotic Gene Cluster
The biosynthetic gene cluster of mersacidin, a lanthionine-containing antimicrobial peptide, is located on the chromosome of the producer, Bacillus sp.
Antimicrobial peptides of lactic acid bacteria: mode of action, genetics and biosynthesis.
The role of the leader peptides is outlined and a conceptual model for bacteriocin maturation is proposed, with emphasis on pore formation in the cytoplasmatic membrane.
Spontaneous resistance in Lactococcus lactis IL1403 to the lantibiotic lacticin 3147.
Cross-resistance to other lantibiotics such as nisin and lacticin 481 was observed, as was cross-res resistance to environmental stresses such as salt, and reduced adsorption of the bacteriocin in to the cell was documented for all resistant mutants.
Bacteriocins of gram-positive bacteria.
In recent years, a group of antibacterial proteins produced by gram-positive bacteria have attracted great interest in their potential use as food preservatives and as antibacterial agents to combat
Common Mechanistic Action of Bacteriocins from Lactic Acid Bacteria
It is suggested that the bacteriocins of lactic acid bacteria share a common mechanism, the depletion of PMF, as has been shown for nisin.
Enhanced Production of Pediocin PA-1 and Coproduction of Nisin and Pediocin PA-1 by Lactococcus lactis
The genetic determinants required for production of pediocin PA-1 were introduced into the nisin-producing strain L. lactis FI5876, where both pediOCinPA-1 and nisin A were simultaneously produced.