Bacterially expressed dsRNA protects maize against SCMV infection

  title={Bacterially expressed dsRNA protects maize against SCMV infection},
  author={Defang Gan and Jiao Zhang and Haibo Jiang and Tong Jiang and Suwen Zhu and Beijiu Cheng},
  journal={Plant Cell Reports},
RNA interference (RNAi) is a sequence-specific, posttranscriptional gene silencing (PTGS) process in plants that is mediated by dsRNA homologous to the silenced gene(s). In this study, we report an efficient method to produce dsRNA using a bacterial expression system. Two fragments of the Sugarcane Mosaic Virus (SCMV) CP (coat protein) gene were amplified by RT-PCR, and cloned into the inverted-repeat cloning vector pUCCRNAi. The two recombinant plasmids were transformed individually into E… CONTINUE READING


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