Bacterial two hybrid: a versatile one-step intracellular selection method.

Abstract

Many antibody fragments, selected ex vivo by phage display, fail to form functional antigen-binding entities when expressed and used intracellularly (i.e., as intrabodies) because the interior of the cell poses significant challenges on the folding of antibodies. Such dropout can be avoided by employing intracellular selection methods like yeast or… (More)
DOI: 10.1007/978-1-61779-968-6_9

Topics

Cite this paper

@article{Pellis2012BacterialTH, title={Bacterial two hybrid: a versatile one-step intracellular selection method.}, author={Mireille Pellis and Serge Muyldermans and C{\'e}cile Vincke}, journal={Methods in molecular biology}, year={2012}, volume={911}, pages={135-50} }